Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3 | Zendy

Charline Jansch | Zendy; Katharina Günther | Zendy; Jonas Waider | Zendy; Georg Ziegler | Zendy; Andrea Forero | Zendy; Sina Kollert | Zendy; Evgeniy Svirin | Zendy; Dirk Pühringer | Zendy; Chee Keong Kwok | Zendy; Reinhard Ullmann | Zendy; Anna Maierhofer | Zendy; Julia Flunkert | Zendy; Thomas Haaf | Zendy; Frank Edenhofer | Zendy; KlausPeter Lesch | Zendy

Open Access

Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3

Author(s) -

Charline Jansch,

Katharina Günther,

Jonas Waider,

Georg Ziegler,

Andrea Forero,

Sina Kollert,

Evgeniy Svirin,

Dirk Pühringer,

Chee Keong Kwok,

Reinhard Ullmann,

Anna Maierhofer,

Julia Flunkert,

Thomas Haaf,

Frank Edenhofer,

KlausPeter Lesch

Publication year - 2018

Publication title -

stem cell research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.654

H-Index - 65

eISSN - 1876-7753

pISSN - 1873-5061

DOI - 10.1016/j.scr.2018.02.005

Subject(s) - induced pluripotent stem cell , biology , glut3 , sendai virus , gene duplication , germ layer , attention deficit hyperactivity disorder , karyotype , microbiology and biotechnology , gene , genetics , glucose transporter , embryonic stem cell , endocrinology , medicine , chromosome , psychiatry , glut1 , insulin

Fibroblasts were isolated from a skin biopsy of a clinically diagnosed 51-year-old female attention-deficit/hyperactivity disorder (ADHD) patient carrying a duplication of SLC2A3, a gene encoding neuronal glucose transporter-3 (GLUT3). Patient fibroblasts were infected with Sendai virus, a single-stranded RNA virus, to generate transgene-free human induced pluripotent stem cells (iPSCs). SLC2A3-D2-iPSCs showed expression of pluripotency-associated markers, were able to differentiate into cells of the three germ layers in vitro and had a normal female karyotype. This in vitro cellular model can be used to study the role of risk genes in the pathogenesis of ADHD, in a patient-specific manner.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research