Generation of urine iPS cell lines from patients with Attention Deficit Hyperactivity Disorder (ADHD) using a non-integrative method | Zendy

Jaroslaw Sochacki | Zendy; Sylvie Devalle | Zendy; Marcelo Reis | Zendy; Paulo Mattos | Zendy; Stevens K. Rehen | Zendy

Open Access

Generation of urine iPS cell lines from patients with Attention Deficit Hyperactivity Disorder (ADHD) using a non-integrative method

Author(s) -

Jaroslaw Sochacki,

Sylvie Devalle,

Marcelo Reis,

Paulo Mattos,

Stevens K. Rehen

Publication year - 2016

Publication title -

stem cell research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.654

H-Index - 65

eISSN - 1876-7753

pISSN - 1873-5061

DOI - 10.1016/j.scr.2016.05.015

Subject(s) - reprogramming , induced pluripotent stem cell , sendai virus , sox2 , klf4 , attention deficit hyperactivity disorder , biology , urine , attention deficit , cell , clinical psychology , endocrinology , immunology , embryonic stem cell , psychology , genetics , virus , gene

Urine samples were collected from three patients (2 males, 1 female) with clinically diagnosed Attention Deficit Hyperactivity Disorder (ADHD) according to DSM-5 criteria using semi-structured interviews (K-SADS adapted for adults) by a trained psychiatrist. Urine epithelial cell lines were established and expanded for subsequent reprogramming procedure. Induced pluripotent stem cells (iPSCs) were derived using integration-free CytoTune®-iPS 2.0 Sendai Reprogramming Kit, which includes Sendai virus particles of the four Yamanaka factors Oct3/4, Sox2, Klf4 and c-Myc.

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