Induced neural stem cells from distinct genetic backgrounds exhibit different reprogramming status | Zendy

Sung Min Kim | Zendy; Kyungtae Lim | Zendy; Tae Hwan Kwak | Zendy; Seung Chan Lee | Zendy; Jung Hyun Im | Zendy; Sai Hali | Zendy; Seon In Hwang | Zendy; Dajeong Kim | Zendy; Jeongho Hwang | Zendy; Kee-Pyo Kim | Zendy; HakJae Chung | Zendy; Jeong Beom Kim | Zendy; Kinarm Ko | Zendy; HyungMin Chung | Zendy; Hoon Taek Lee | Zendy; Hans R. Schöler | Zendy; DongWook Han | Zendy

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Induced neural stem cells from distinct genetic backgrounds exhibit different reprogramming status

Author(s) -

Sung Min Kim,

Kyungtae Lim,

Tae Hwan Kwak,

Seung Chan Lee,

Jung Hyun Im,

Sai Hali,

Seon In Hwang,

Dajeong Kim,

Jeongho Hwang,

Kee-Pyo Kim,

HakJae Chung,

Jeong Beom Kim,

Kinarm Ko,

HyungMin Chung,

Hoon Taek Lee,

Hans R. Schöler,

DongWook Han

Publication year - 2016

Publication title -

stem cell research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.654

H-Index - 65

eISSN - 1876-7753

pISSN - 1873-5061

DOI - 10.1016/j.scr.2016.02.025

Subject(s) - reprogramming , biology , somatic cell , ectopic expression , gene silencing , microbiology and biotechnology , genetics , transgene , neural stem cell , lineage (genetic) , transcription factor , somatic fusion , cell culture , stem cell , cell , gene

Somatic cells could be directly converted into induced neural stem cells (iNSCs) by ectopic expression of defined transcription factors. However, the underlying mechanism of direct lineage transition into iNSCs is largely unknown. In this study, we examined the effect of genetic background on the direct conversion process into an iNSC state. The iNSCs from two different mouse strains exhibited the distinct efficiency of lineage conversion as well as clonal expansion. Furthermore, the expression levels of endogenous NSC markers, silencing of transgenes, and in vitro differentiation potential were also different between iNSC lines from different strains. Therefore, our data suggest that the genetic background of starting cells influences the conversion efficiency as well as reprogramming status of directly converted iNSCs.

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