Modification of Gold Electrodes with Bacterial Reaction Centres Immobilized by Laser Induced Forward Transfer (LIFT) Technique for Amperometric Herbicide Detection
Author(s) -
Maria Rachele Guascito,
Marianneza Chatzipetrou,
Daniela Chirizzi,
Massimo Trotta,
Maria Massaouti,
Livia Giotta,
Francesco Milano,
I. Zergioti
Publication year - 2017
Publication title -
procedia technology
Language(s) - English
Resource type - Journals
ISSN - 2212-0173
DOI - 10.1016/j.protcy.2017.04.083
Subject(s) - rhodobacter sphaeroides , electron transfer , electrode , amperometry , surface modification , laser , materials science , electrochemiluminescence , optoelectronics , fabrication , colloidal gold , biosensor , nanotechnology , electrochemistry , chemistry , photochemistry , optics , nanoparticle , biochemistry , medicine , photosynthesis , physics , alternative medicine , pathology
The functionalization of screen-printed electrodes (SPEs) with a thin film of reaction centre (RC) proteins from the phototrophic bacterium Rhodobacter (R.) sphaeroides, by means of laser induced forward transfer (LIFT) technique, allowed the fabrication of robust and sensitive bio-hybrid devices for terbutryn detection and analysis. The optimal wiring between RCs and the gold electrode surface, achieved by LIFT, led to the generation of cathodic photocurrents sustained by a direct electron transfer (DET) mechanism, which were attenuated by addition of the herbicide inhibitor.
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