Multiplex Detection of Biothreat Agents Using an Automated Electrochemical ELISA Platform | Zendy

Christopher Pöhlmann | Zendy; Laurent Bellanger | Zendy; Michal Dřevínek | Zendy; Thomas Elßner | Zendy

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Multiplex Detection of Biothreat Agents Using an Automated Electrochemical ELISA Platform

Author(s) -

Christopher Pöhlmann,

Laurent Bellanger,

Michal Dřevínek,

Thomas Elßner

Publication year - 2017

Publication title -

procedia technology

Language(s) - English

Resource type - Journals

ISSN - 2212-0173

DOI - 10.1016/j.protcy.2017.04.045

Subject(s) - biochip , multiplex , identification (biology) , virology , microbiology and biotechnology , nanotechnology , biology , materials science , bioinformatics , botany

Modern threats of bioterrorism force the need to immediately identify biothreat agents. Here, we present an electrochemical detection platform for identification of six biothreat agents in parallel within approx. 20 minutes in an automated procedure. The detection platform is permanently housed in a robust, lightweight suitcase offering a fully deconable housing. Limits of detection are in the range of 103 - 105 colony forming units per mL or 104 - 105 plaque forming units per mL for bacterial or viral agents, respectively. These results demonstrate the potential of electrochemical biochips for parallel and sensitive on-site detection of biothreat agents.

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