Immunogenicity and Specificity of Anti recombinant Protein Fim-C-Salmonella typhimurium Antibody as a Model to Develop Typhoid Vaccine | Zendy

Muktiningsih Nurjayadi | Zendy; Dea Apriyani | Zendy; Umar Hasan | Zendy; Imam Santoso | Zendy; Fera Kurniadewi | Zendy; Irma Ratna Kartika | Zendy; Kurnia Agustini | Zendy; Fernita Puspasari | Zendy; Dessy Natalia | Zendy; Wibowo Mangunwardoyo | Zendy

Open Access

Immunogenicity and Specificity of Anti recombinant Protein Fim-C-Salmonella typhimurium Antibody as a Model to Develop Typhoid Vaccine

Author(s) -

Muktiningsih Nurjayadi,

Dea Apriyani,

Umar Hasan,

Imam Santoso,

Fera Kurniadewi,

Irma Ratna Kartika,

Kurnia Agustini,

Fernita Puspasari,

Dessy Natalia,

Wibowo Mangunwardoyo

Publication year - 2016

Publication title -

procedia chemistry

Language(s) - English

Resource type - Journals

ISSN - 1876-6196

DOI - 10.1016/j.proche.2016.01.037

Subject(s) - immunogenicity , recombinant dna , typhoid fever , salmonella , antibody , virology , microbiology and biotechnology , antigen , salmonella typhi , biology , escherichia coli , immunology , bacteria , gene , biochemistry , genetics

almonella typhimurium, that cause typhoid fever in mice was used as a model to study typhoid fever in humans. This study is aimed to determine the immunogenicity of recombinant protein of Fim-C-S. typhimurium and specificity of antibody anti-recombinant protein of Fim-C-S. typhimurium in ddY mice. The immunogenicity studies by ELISA indicated that recombinant protein of Fim-C-S. typhimurium evokes strong immune response in ddY mice, particularly in experiment group. The specificity evaluated by Western Immunobloting technique indicated that the antibody anti-recombinant protein of Fim-C-S. typhimurium can detect the 31 kDa of recombinant protein of Fim-C-S. typhimurium as an antigen. This data reported that recombinant protein of Fim-C-S. typhimurium is immunogenic and the antibody anti-recombinant protein of Fim-C-S. typhimurium is specific. The results of this study can be used as a model to develop recombinant vaccine candidate against typhoid

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