Open AccessEosinophilic Allergic Polyp: A Clinically Oriented Approach
Author(s) -
Kim Kwang Hyun,
Han Doo Hee,
Lee Chul Hee,
Min YangGi,
Rhee ChaeSeo
Publication year - 2010
Publication title -
otolaryngology–head and neck surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.232
H-Index - 121
eISSN - 1097-6817
pISSN - 0194-5998
DOI - 10.1016/j.otohns.2010.06.608
Subject(s) - citation , medicine , philosophy , library science , computer science
and after oral GC therapy (GC-treated) and 8 controls of inferior turbinate mucosa were included. cDNA microarray analysis, together with histoand immunohistochemical validation, was performed. RESULTS: Infiltration of eosinophils and neutrophils was prominent in GC-nave NP. GC was effective in reducing the recruitment of both nasal mucosal eosinophils and neutrophils. Thirty-one eosinophil-associated genes were differentially expressed in GC-nave NP versus control. Expression of five genes (MMP9, NR4A1, NR4A2, CD69 and DUSP1) demonstrated a significant change after GC treatment. On the other hand, expressions of 14 neutrophil-associated genes were different in GC-nave NP versus control and 3 of them (MMP7, MMP9 and CXCL2) were responsive to GC therapy. Immuno-staining showed the co-localization of MMP7 and MMP9 with neutrophils and the decrease of these proteins in NP after GC treatment. NR4A1/NR4A2 proteins were absent in eosinophils from all GC-nave NP but present in some GC-treated NP. CONCLUSION: GC is effective in inhibiting both eosinophil and neutrophil infiltration in NP, which can be demonstrated by modulation of those important eosinophiland neutrophilassociated genes.