Open AccessA method for identification of vocal fold lamina propria fibroblasts in culture
Author(s) -
Thibeault Susan L.,
Li Wenhua,
Bartley Stephanie
Publication year - 2008
Publication title -
otolaryngology–head and neck surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.232
H-Index - 121
eISSN - 1097-6817
pISSN - 0194-5998
DOI - 10.1016/j.otohns.2008.09.009
Subject(s) - fibroblast , cytokeratin , lamina propria , cell culture , biology , von willebrand factor , basic fibroblast growth factor , microbiology and biotechnology , doubling time , immortalised cell line , in vitro , immunology , epithelium , pathology , immunohistochemistry , growth factor , medicine , genetics , platelet , receptor
Objective Vocal fold biology research is emerging as a vital area of study in laryngology. One impediment is the lack of both commercially available vocal fold lamina propria fibroblasts and a constitutively expressed specific marker for fibroblasts. We present an in vitro technique that allows for identification of fibroblasts by ruling out the possibility of the cells belonging to other lineages that are found in vocal fold tissue. Study Design An in vitro study. Methods Two primary vocal fold fibroblast cell lines and one immortalized vocal fold fibroblast cell line were cultured. Immunohistologic staining for α‐actinin, cytokeratin 19, and von Willebrand factor was completed for the three fibroblast lines in addition to skeletal, endothelial, and epithelial cell lines. Cell type was differentiated by positive staining for α‐actinin, cytokeratin 19, and von Willebrand factor. Results Fibroblast cultures did not express α‐actinin, cytokeratin 19, and von Willebrand factor, whereas skeletal muscle, endothelial, and epithelial cultured cells expressed each respectively. Conclusions This simple rule‐out methodology for fibroblast confirmation is an important step when establishing cell culture, and it establishes sound internal validity particularly in the early stages of this emerging area of study.