Open AccessExperimentally induced phonation increases matrix metalloproteinase‐1 gene expression in normal rabbit vocal fold
Author(s) -
Rousseau Bernard,
Ge PingJiang,
French Lesley C.,
Zealear David L.,
Thibeault Susan L.,
Ossoff Robert H.
Publication year - 2008
Publication title -
otolaryngology–head and neck surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.232
H-Index - 121
eISSN - 1097-6817
pISSN - 0194-5998
DOI - 10.1016/j.otohns.2007.10.024
Subject(s) - phonation , rabbit (cipher) , fold (higher order function) , vocal folds , gene , matrix metalloproteinase , microbiology and biotechnology , vocal fold paralysis , biology , chemistry , anatomy , medicine , larynx , genetics , audiology , mathematics , surgery , computer science , paralysis , statistics , programming language
Objectives An in vivo rabbit model was used to study the effect of 3 hours of experimentally induced phonation on messenger RNA expression of the normal vocal fold. Study Design Prospective; animal model. Subjects and Methods Ten rabbits received experimental phonation for 3 hours, followed by 1 hour of recovery. A separate group of 5 rabbits served as no‐phonation controls. We measured messenger RNA expression of matrix metalloproteinase‐1, MMP‐9, and interleukin‐1β using real‐time reverse‐transcribed polymerase chain reaction. Gene expression ratios from phonation and control animals were assessed with the Mann‐Whitney U test. Results Phonation (77 ± 3 dB; 429 ± 141 Hz) resulted in increased matrix metalloproteinase‐1 gene expression from rabbits receiving experimental phonation compared with controls, and a nonsignificant increase in matrix metalloproteinase‐9 and interleukin‐1β gene expression. Conclusion Matrix metalloproteinases play a role in maintaining tissue homeostasis. Investigation of cellular responses to experimental phonation may provide insight into how matrix metalloproteinases and other extracellular matrices contribute to maintenance of the vocal fold and development of pathology.