Impact of Dental Pulp Stem Cells Overexpressing Hepatocyte Growth Factor after Cerebral Ischemia/Reperfusion in Rats | Zendy

Kota Sowa | Zendy; Chikako Nito | Zendy; Masataka Nakajima | Zendy; Satoshi Suda | Zendy; Yasuhiro Nishiyama | Zendy; Yuki Sakamoto | Zendy; Yuko NitaharaKasahara | Zendy; Aki Nakamura-Takahashi | Zendy; Masayuki Ueda | Zendy; Kazumi Kimura | Zendy; Takashi Okada | Zendy

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Impact of Dental Pulp Stem Cells Overexpressing Hepatocyte Growth Factor after Cerebral Ischemia/Reperfusion in Rats

Author(s) -

Kota Sowa,

Chikako Nito,

Masataka Nakajima,

Satoshi Suda,

Yasuhiro Nishiyama,

Yuki Sakamoto,

Yuko NitaharaKasahara,

Aki Nakamura-Takahashi,

Masayuki Ueda,

Kazumi Kimura,

Takashi Okada

Publication year - 2018

Publication title -

molecular therapy — methods and clinical development

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.285

H-Index - 32

ISSN - 2329-0501

DOI - 10.1016/j.omtm.2018.07.009

Subject(s) - hepatocyte growth factor , hepatocyte , dental pulp stem cells , ischemia , stem cell , medicine , microbiology and biotechnology , chemistry , biology , biochemistry , in vitro , receptor

Hepatocyte growth factor (HGF) has neuroprotective effects against ischemia-induced injuries. Dental pulp stem cell (DPSC) transplantation attenuates tissue injury in the brain of rats with post-transient middle cerebral artery occlusion. We sought to determine whether DPSCs that overexpress HGF can enhance their therapeutic effects on brain damage post-ischemia/reperfusion injury. Treatment with DPSCs overexpressing HGF reduced infarct volumes compared to unmodified DPSC treatment at 3 and 7 days post-transient middle cerebral artery occlusion. The use of unmodified DPSCs and DPSCs overexpressing HGF was associated with improved motor function compared to that with administration of vehicle at 7 days post-transient middle cerebral artery occlusion. DPSCs overexpressing HGF significantly inhibited microglial activation and pro-inflammatory cytokine production along with suppression of neuronal degeneration. Post-reperfusion, DPSCs overexpressing HGF attenuated the decreases in tight junction proteins, maintained blood-brain barrier integrity, and increased microvessel density in peri-infarct areas. The administration of DPSCs overexpressing HGF during the acute phase of stroke increased their neuroprotective effects by modulating inflammation and blood-brain barrier permeability, thereby promoting improvements in post-ischemia/reperfusion brain injury.

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