Open AccessThe detection of feline coronaviruses in blood samples from cats by mRNA RT-PCR
Author(s) -
Kezban Can-Şahna,
Veysel Soydal Ataseven,
Dilek Pınar,
Tuba Çiğdem Oğuzoğlu
Publication year - 2007
Publication title -
journal of feline medicine and surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.837
H-Index - 61
eISSN - 1532-2750
pISSN - 1098-612X
DOI - 10.1016/j.jfms.2007.03.002
Subject(s) - cats , feline infectious peritonitis , virology , real time polymerase chain reaction , medicine , coronavirus , polymerase chain reaction , pathology , biology , infectious disease (medical specialty) , covid-19 , disease , gene , biochemistry
In this study, 26 blood samples were collected from 25 healthy cats and one cat with clinical signs suggestive of feline infectious peritonitis (FIP), namely, fever, weight loss, enlarged abdomen, and ascites. Blood samples were tested for feline coronavirus (FCoV) messenger RNA (mRNA) by an reverse transcriptase-polymerase chain reaction (RT-PCR) assay which has previously been described to have a high specificity in the diagnosis of clinical FIP [Simons AF, Vennema H, Rofina JE, Pol JM, Horzinek MC, Rottier PJM, Egberink HF (2005) A mRNA PCR for the diagnosis of feline infectious peritonitis. Journal of Virological Methods124, 111-116]. Overall we found 14 (54%) of the cats were positive for FCoV including the cat with clinical disease, but the high rate of positivity among healthy cats suggested a poor specificity for the clinical diagnosis of FIP among these cats. It was observed that the positivity rate was highest in cats aged between 6 months-1 year old. Our findings suggest that FCoVs may be present in the blood samples from healthy cats as well as cats with clinical FIP.
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