Open AccessLaserspray ionization (LSI) ion mobility spectrometry (IMS) mass spectrometry
Author(s) -
Ellen D. Inutan,
Sarah Trimpin
Publication year - 2010
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/j.jasms.2010.03.039
Subject(s) - chemistry , mass spectrometry , ion mobility spectrometry , ion , ionization , analytical chemistry (journal) , ambient ionization , atmospheric pressure laser ionization , ion source , time of flight mass spectrometry , ion mobility spectrometry–mass spectrometry , sample preparation in mass spectrometry , thermal ionization mass spectrometry , tandem mass spectrometry , soft laser desorption , matrix assisted laser desorption/ionization , chemical ionization , electron ionization , desorption , selected reaction monitoring , chromatography , electrospray ionization , organic chemistry , adsorption
A simple device is described for desolvation of highly charged matrix/analyte clusters produced by laser ablation leading to multiply charged ions that are analyzed by ion mobility spectrometry-mass spectrometry. Thus, for example, highly charged ions of ubiquitin and lysozyme are cleanly separated in the gas phase according to size and mass (shape and molecular weight) as well as charge using Tri-Wave ion mobility technology coupled to mass spectrometry. This contribution confirms the mechanistic argument that desolvation is necessary to produce multiply charged matrix-assisted laser desorption/ionization (MALDI) ions and points to how these ions can be routinely formed on any atmospheric pressure mass spectrometer.
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