Open AccessGeneration of CsI cluster ions for mass calibration in matrix-assisted laser desorption/ionization mass spectrometry
Author(s) -
Xianwen Lou,
Joost L. J. van Dongen,
E. W. Meijer
Publication year - 2010
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/j.jasms.2010.02.029
Subject(s) - chemistry , mass spectrometry , ion , reflectron , soft laser desorption , analytical chemistry (journal) , ionization , cluster (spacecraft) , ion source , matrix assisted laser desorption electrospray ionization , matrix assisted laser desorption/ionization , thermal ionization mass spectrometry , desorption , time of flight mass spectrometry , chromatography , organic chemistry , adsorption , computer science , programming language
A simple method was developed for the generation of cesium iodide (CsI) cluster ions up to m/z over 20,000 in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Calibration ions in both positive and negative ion modes can readily be generated from a single MALDI spot of CsI(3) with 2-[(2E)-3-(4-tert-butylphenyl)-2-methylprop-2-enylidene] malononitrile (DCTB) matrix. The major cluster ion series observed in the positive ion mode is [(CsI)(n)Cs](+), and in the negative ion mode is [(CsI)(n)I](-). In both cluster series, ions spread evenly every 259.81 units. The easy method described here for the production of CsI cluster ions should be useful for MALDI MS calibrations.