Open AccessComplementary peptide sequence coverage using alternative enzymes for on-line digestion with a triaxial electrospray probe
Author(s) -
Andrew B. Dykstra,
Maolian Chen,
Kelsey D. Cook
Publication year - 2009
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/j.jasms.2009.07.022
Subject(s) - chemistry , digestion (alchemy) , electrospray , enzyme , pepsin , peptide , chromatography , substrate (aquarium) , dissolution , sequence (biology) , proteolytic enzymes , mass spectrometry , biochemistry , organic chemistry , oceanography , geology
Using alternative enzymes for on-line digestion with a triaxial electrospray probe extends sequence coverage. This is the first report of utilization of our triaxial probe for on-line analysis with enzymes other than pepsin, suggesting potential for broader application. The probe allows access to processes occurring on a timescale and/or involving substrate conformations complementary to those for conventional (off-line) digestion. Some of the features observed in application to Abeta fibrils are suggestive of unique reactive intermediates during dissolution. Data obtained with enzyme mixtures suggest synergistic effects.