Open AccessEvaluation of the ALiPHAT method for PC-IDMS and correlation of limits-of-detection with nonpolar surface area
Author(s) -
D. Keith Williams,
Daniel L. Comins,
J. L. Whitten,
David C. Muddiman
Publication year - 2009
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/j.jasms.2009.07.019
Subject(s) - chemistry , reagent , chromatography , detection limit , peptide , electrospray ionization , alkylation , mass spectrometry , biochemistry , organic chemistry , catalysis
PC-IDMS experiments for two peptides, laminin nonapeptide and the N-terminal tryptic peptide of prostate specific antigen, were performed utilizing a variety of alkylating reagents. These experiments were conducted to investigate how hydrophobicity influences the limits-of-detection (LOD) by altering their electrospray ionization response. Nonpolar surface areas were calculated for both peptides and all alkylating reagents to provide an estimate of the hydrophobicity of the differently alkylated peptides. Decreases in LOD by 2-fold were observed for both peptides between the best and worst performing combination of alkylating reagent. However, while an increase in hydrophobicity was found to aid in decreasing LOD to an extent, beyond a certain hydrophobicity, we observed a decrease.