Open AccessSpectroscopic evidence for mobilization of amide position protons during CID of model peptide ions
Author(s) -
Samuel P. Molesworth,
Christopher M. Leavitt,
Gary S. Groenewold,
Jos Oomens,
Jeffrey D. Steill,
Michael J. Van Stipdonk
Publication year - 2009
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/j.jasms.2009.06.007
Subject(s) - infrared multiphoton dissociation , chemistry , oxazolone , protonation , fragmentation (computing) , amide , dissociation (chemistry) , photochemistry , pyridine , ion , spectroscopy , infrared spectroscopy , ring (chemistry) , stereochemistry , medicinal chemistry , organic chemistry , physics , quantum mechanics , computer science , operating system
Infrared multiple photon dissociation (IRMPD) spectroscopy was used to study formation of b2+ from nicotinyl-glycine-glycine-methyl ester (NicGGOMe). IRMPD shows that NicGGOMe is protonated at the pyridine ring of the nicotinyl group, and more importantly, that b2+ from NicGGOMe is not protonated at the oxazolone ring, as would be expected if the species were generated on the conventional bn+/yn+ oxazolone pathway, but at the pyridine ring instead. IRMPD data support a hypothesis that formation of b2+ from NicGGOMe involves mobilization and transfer of an amide position proton during the fragmentation reaction.