Open AccessThe measurement of charge for induction-based fluidic MALDI dispense event and nanoliter volume verification in real time
Author(s) -
Brent Hilker,
Kevin J. Clifford,
Andrew D. Sauter,
Andrew D. Sauter,
Julie P. Harmon
Publication year - 2009
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/j.jasms.2009.01.015
Subject(s) - chemistry , fluidics , volume (thermodynamics) , event (particle physics) , analytical chemistry (journal) , charge (physics) , chromatography , nanotechnology , materials science , physics , electrical engineering , quantum mechanics , engineering
This study preliminarily investigates whether nanoliter volumes of concentrated polar liquids and organic monomers launched to targets using induction based fluidics (IBF) can be verified through the real time charge measurements. We show that using a nanoliter IBF dispensing device and nanocoulomb meter, charge measurements made on nanoliter drops in real time are correlated with surface area following Gauss's Law. We infer the "induction only" formation of the double layer showing the ability to determine nanoliter volumes, nearly instantaneously, in real time. We discuss the implications that these observations may have for on improving/monitoring MALDI quantitation and its quality control.