Open AccessA linear ion trap mass spectrometer with versatile control and data acquisition for ion/ion reactions
Author(s) -
Matthew W. Soyk,
Q. Zhao,
R. S. Houk,
Ethan R. Badman
Publication year - 2008
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/j.jasms.2008.08.020
Subject(s) - chemistry , ion , mass spectrometry , ion trap , ion source , quadrupole ion trap , ion trapping , ambient ionization , ionization , analytical chemistry (journal) , selected reaction monitoring , electrospray ionization , chemical ionization , tandem mass spectrometry , chromatography , organic chemistry
A linear ion trap (LIT) with electrospray ionization (ESI) for top-down protein analysis has been constructed. An independent atmospheric sampling glow discharge ionization (ASGDI) source produces reagent ions for ion/ion reactions. The device is also meant to enable a wide variety of ion/ion reaction studies. To reduce the instrument's complexity and make it available for wide dissemination, only a few simple electronics components were custom built. The instrument functions as both a reaction vessel for gas-phase ion/ion reactions and a mass spectrometer using mass-selective axial ejection. Initial results demonstrate trapping efficiency of 70% to 90% and the ability to perform proton transfer reactions on intact protein ions, including dual polarity storage reactions, transmission mode reactions, and ion parking.