Tandem MS can distinguish hyaluronic acid from N-acetylheparosan | Zendy

Zhenqing Zhang | Zendy; Jin Xie | Zendy; Jian Liu | Zendy; Robert J. Linhardt | Zendy

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Tandem MS can distinguish hyaluronic acid from N-acetylheparosan

Author(s) -

Zhenqing Zhang,

Jin Xie,

Jian Liu,

Robert J. Linhardt

Publication year - 2007

Publication title -

journal of the american society for mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.961

H-Index - 127

eISSN - 1879-1123

pISSN - 1044-0305

DOI - 10.1016/j.jasms.2007.10.012

Subject(s) - chemistry , glycosidic bond , tandem mass spectrometry , disaccharide , cleavage (geology) , mass spectrometry , fast atom bombardment , hyaluronic acid , stereochemistry , oligosaccharide , glucuronic acid , glycosylation , chromatography , biochemistry , enzyme , polysaccharide , geotechnical engineering , fracture (geology) , biology , engineering , genetics

Isobaric oligosaccharides enzymatically prepared from hyaluronic acid (HA) and N-acetylheparosan (NAH), were distinguished using tandem mass spectrometry. The only difference between the two series of oligosaccharides was the linkage pattern (in HA 1-->3 and in NAH 1-->4) between glucuronic acid and N-acetylglucosamine residues. Tandem mass spectrometry afforded spectra in which glycosidic cleavage fragment ions were observed for both HA and NAH oligosaccharides. Cross-ring cleavage ions 0,2An and 0,2An-h (n is even number) were observed only in GlcNAc residues of NAH oligosaccharides. One exception was an 0,2A2 ion fragment observed for the disaccharide from HA. These cross-ring cleavage fragment ions are useful to definitively distinguish HA and NAH oligosaccharides.

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