Open AccessA computer algorithm for the identification of protein interactions from the spectra of masses (PRISM)
Author(s) -
Joshua W. K. Ho,
Bethny Morrissey,
Kevin M. Downard
Publication year - 2007
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/j.jasms.2006.11.005
Subject(s) - chemistry , prism , mass spectrum , spectral line , graphical user interface , mass spectrometry , identification (biology) , algorithm , analytical chemistry (journal) , simple (philosophy) , interface (matter) , biological system , chromatography , computer science , biochemistry , physics , optics , pulmonary surfactant , philosophy , botany , gibbs isotherm , epistemology , astronomy , biology , programming language
A new algorithm is reported to assist with the identification of protein interaction domains by comparing pairs of MALDI mass spectra recorded for protein digests treated with a binding partner versus an untreated control. Known as PRISM, for protein interactions from the spectra of masses, the algorithm imports m/z versus peak area data directly from a pair of MALDI mass spectra recorded for the control and reaction sample. The algorithm is shown to be able to successfully identify antigenic determinants for protein antigens within mixed protein digests. The algorithm has general utility for the comparative analysis of differences within any two mass spectra of any type and is easily implemented using a simple, intuitive graphical user interface (GUI).