MALDI analysis of oligonucleotides directly from montmorillonite | Zendy

Dmitri V. Zagorevskii | Zendy; Michael F. Aldersley | Zendy; James P. Ferris | Zendy

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MALDI analysis of oligonucleotides directly from montmorillonite

Author(s) -

Dmitri V. Zagorevskii,

Michael F. Aldersley,

James P. Ferris

Publication year - 2006

Publication title -

journal of the american society for mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.961

H-Index - 127

eISSN - 1879-1123

pISSN - 1044-0305

DOI - 10.1016/j.jasms.2006.05.012

Subject(s) - chemistry , montmorillonite , oligonucleotide , chromatography , catalysis , mass spectrometry , molecular mass , matrix (chemical analysis) , organic chemistry , biochemistry , dna , enzyme

Oligonucleotides synthesized on a montmorillonite catalyst were analyzed directly. By mixing the catalyst with a matrix (2,4,6-trihydroxyacetophenone or 6-aza-2-thiothymine) and dibasic ammonium citrate, higher molecular weight products were detected compared with "classical" methods such as gel electrophoresis and HPLC with UV as a detector. The oligomers (30-mers and higher) were detected by mass spectrometry even though their concentration was less than 10(-4)% of the total content of the RNA. This method is different from the (MALDI) analysis of the eluates from montmorillonite, which otherwise requires desalting. Placing reaction mixtures with a high concentration of buffers on homoionic, preferably Li-containing, montmorillonite does not require desalting.

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