Open AccessA parallel approach to post source decay MALDI-TOF analysis
Author(s) -
Daniel J. Kenny,
Jeffery M. Brown,
Martin Palmer,
Marten F. Snel,
Robert Bateman
Publication year - 2006
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/j.jasms.2005.09.006
Subject(s) - reflectron , chemistry , ion , mass spectrometry , ionization , mass spectrum , analytical chemistry (journal) , fragment (logic) , ion source , matrix assisted laser desorption/ionization , spectral line , time of flight mass spectrometry , desorption , chromatography , physics , algorithm , organic chemistry , adsorption , astronomy , computer science
We present a novel enhancement to matrix-assisted laser desorption ionization (MALDI) post-source decay (PSD) analysis whereby fragment ions from multiple precursor ions are acquired into the same spectrum without employing a timed ion gate to preselect each parent ion. Fragment ions are matched to their corresponding precursor ions by comparing spectra acquired at slightly different reflectron electric fields. By measuring the difference in time-of-flight (TOF) between the two spectra for each fragment, it is possible to calculate the mass of the fragment ion and its parent. This new "parallel PSD" technique reduces analysis time and consumes less sample than conventional PSD, which requires an ion gate for serial preselection of precursor ions.