Open AccessSensitive LC MS quantitative analysis of carbohydrates by Cs+ attachment
Author(s) -
Eduard Rogatsky,
Harsha Jayatillake,
Gayotri Goswami,
Vlad Tomuta,
Daniel T. Stein
Publication year - 2005
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/j.jasms.2005.07.017
Subject(s) - chemistry , chromatography , mass spectrometry , derivatization , electrospray ionization , selected reaction monitoring , isotope dilution , electrospray , carbohydrate , sample preparation , analytical chemistry (journal) , chemical ionization , ionization , tandem mass spectrometry , ion , organic chemistry
The development of a sensitive assay for the quantitative analysis of carbohydrates from human plasma using LC/MS/MS is described in this paper. After sample preparation, carbohydrates were cationized by Cs(+) after their separation by normal phase liquid chromatography on an amino based column. Cesium is capable of forming a quasi-molecular ion [M + Cs](+) with neutral carbohydrate molecules in the positive ion mode of electrospray ionization mass spectrometry. The mass spectrometer was operated in multiple reaction monitoring mode, and transitions [M + 133] --> 133 were monitored (M, carbohydrate molecular weight). The new method is robust, highly sensitive, rapid, and does not require postcolumn addition or derivatization. It is useful in clinical research for measurement of carbohydrate molecules by isotope dilution assay.