Carbon nanotubes as affinity probes for peptides and proteins in MALDI MS analysis | Zendy

WeiYu Chen | Zendy; Lung-Shen Wang | Zendy; HsinTien Chiu | Zendy; YuChie Chen | Zendy; Chi Young Lee | Zendy

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Carbon nanotubes as affinity probes for peptides and proteins in MALDI MS analysis

Author(s) -

WeiYu Chen,

Lung-Shen Wang,

HsinTien Chiu,

YuChie Chen,

Chi Young Lee

Publication year - 2004

Publication title -

journal of the american society for mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.961

H-Index - 127

eISSN - 1879-1123

pISSN - 1044-0305

DOI - 10.1016/j.jasms.2004.08.001

Subject(s) - chemistry , analyte , carbon nanotube , citric acid , aqueous solution , small molecule , alkali metal , biomolecule , mass spectrometry , chromatography , matrix (chemical analysis) , combinatorial chemistry , nanotechnology , organic chemistry , biochemistry , materials science

Recently, carbon nanotubes (CNTs) have been reported to be an effective MALDI matrix for small molecules (Anal. Chem.2003, 75, 6191). In a somewhat related study, we have employed CNTs produced by using NaH-treated anodic aluminum oxide (Na@AAO) as a reactive template as the assisting matrix for MALDI analysis upon the addition of high concentrations of citrate buffer. Our results indicate that the mass range can be extended to ca. 12,000 Da and that alkali metal adducts of analytes are effectively reduced. Furthermore, we have employed citric acid-treated CNTs as affinity probes to selectively concentrate traces of analytes from aqueous solutions. High concentrations of salts and surfactants in the sample solutions are also tolerated. This approach is very suitable for the MALDI analysis of small proteins, peptides, and protein enzymatic digest products.

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