Low Nanoliter Acoustic Transfer of Aqueous Fluids with High Precision and Accuracy of Volume Transfer and Positional Placement | Zendy

David L. Harris | Zendy; Mitchell W. Mutz | Zendy; M. Sonntag | Zendy; Richard Stearns | Zendy; Jean Shieh | Zendy; Siobhan C. Pickett | Zendy; Richard Ellson | Zendy; Joe Olechno | Zendy

Open Access

Low Nanoliter Acoustic Transfer of Aqueous Fluids with High Precision and Accuracy of Volume Transfer and Positional Placement

Author(s) -

David L. Harris,

Mitchell W. Mutz,

M. Sonntag,

Richard Stearns,

Jean Shieh,

Siobhan C. Pickett,

Richard Ellson,

Joe Olechno

Publication year - 2008

Publication title -

jala journal of the association for laboratory automation

Language(s) - English

Resource type - Journals

eISSN - 1540-2452

pISSN - 1535-5535

DOI - 10.1016/j.jala.2007.12.002

Subject(s) - aqueous solution , reagent , miniaturization , volume (thermodynamics) , chemistry , fluorescence , laboratory flask , transfer (computing) , analytical chemistry (journal) , dimethyl sulfoxide , materials science , chromatography , computer science , thermodynamics , nanotechnology , organic chemistry , optics , physics , parallel computing

The utility of acoustic droplet ejection (ADE), originally used to transfer dimethyl sulfoxide (DMSO) solutions, is expanded beyond the transfer of DMSO to a wide variety of aqueous solutions common to biochemical experiments and assays. Aqueous-based liquids are transferred with high precision (coefficient of variation <5% for volume transfers of 5–50 nL) and accuracy (within 5% of expected volume), similar to that seen with DMSO transfers. The precision and accuracy of the technique are measured via fluorescence. ADE transfers of aqueous solutions may facilitate the miniaturization of assays leading to increased throughput and reduced reagent usage.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research