High-Throughput Protein Crystallography | Zendy

Arezou Azarani | Zendy; Brent W. Segelke | Zendy; Dominique Toppani | Zendy; Timothy Lekin | Zendy

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High-Throughput Protein Crystallography

Author(s) -

Arezou Azarani,

Brent W. Segelke,

Dominique Toppani,

Timothy Lekin

Publication year - 2006

Publication title -

jala journal of the association for laboratory automation

Language(s) - English

Resource type - Journals

eISSN - 1540-2452

pISSN - 1535-5535

DOI - 10.1016/j.jala.2005.09.004

Subject(s) - protein crystallization , automation , software , throughput , crystallization , computer science , computer hardware , embedded system , engineering , operating system , mechanical engineering , chemical engineering , wireless

An automated platform for low-volume, highthroughput protein crystallization by the sitting drop vapor diffusion has been developed. This platform consists of a novel Society of Biomolecular Screening standard microplate technology, standard automation liquid handling equipment, a software to design customizable, random crystallization buffers, an in-house designed scanner, a crystal recognition software, and Laboratory Information Management Systems. These technologies are designed to eliminate obstacles to the production of protein crystals and to address a number of critical issues faced by crystallography laboratories, namely precision and accuracy, ease of integration and use, speed, and cost.

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