Open AccessA new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses
Author(s) -
Sylvia Broeders,
Linda Garlant,
MarieAlice Fraiture,
Els Vandermassen,
Vanessa Suin,
Jessica Vanhomwegen,
Myrielle DupontRouzeyrol,
Dominique Rousset,
Steven Van Gucht,
Nancy H. C. Roosens
Publication year - 2019
Publication title -
international journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 89
eISSN - 1878-3511
pISSN - 1201-9712
DOI - 10.1016/j.ijid.2019.12.028
Subject(s) - chikungunya , taqman , zika virus , virology , outbreak , multiplex , computational biology , biology , virus , real time polymerase chain reaction , bioinformatics , genetics , gene
The re-emergence and spread of tropical viruses to new areas has raised a wave of concern worldwide. In order to treat patients at an early stage and prevent the diffusion of an outbreak, early diagnosis, and therefore fast and adequate detection, is needed. To this end, a multiplex reverse transcription real-time polymerase chain reaction TaqMan method was designed to detect Zika (ZIKV) and chikungunya (CHIKV) viruses simultaneously.
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