Direct identification of microorganisms from positive blood cultures by MALDI-TOF MS using an in-house saponin method | Zendy

Shota Yonetani | Zendy; H. Ohnìshì | Zendy; Kiyofumi Ohkusu | Zendy; Tetsuya Matsumoto | Zendy; Takashi Watanabe | Zendy

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Direct identification of microorganisms from positive blood cultures by MALDI-TOF MS using an in-house saponin method

Author(s) -

Shota Yonetani,

H. Ohnìshì,

Kiyofumi Ohkusu,

Tetsuya Matsumoto,

Takashi Watanabe

Publication year - 2016

Publication title -

international journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.278

H-Index - 89

eISSN - 1878-3511

pISSN - 1201-9712

DOI - 10.1016/j.ijid.2016.09.014

Subject(s) - centrifugation , chromatography , saponin , matrix assisted laser desorption/ionization , bacteria , blood culture , chemistry , mass spectrometry , sample preparation , microbiology and biotechnology , biology , medicine , desorption , biochemistry , antibiotics , pathology , alternative medicine , organic chemistry , adsorption , genetics

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast and reliable method for the identification of bacteria. A MALDI Sepsityper kit is generally used to prepare samples obtained directly from culture bottles. However, the relatively high cost of this kit is a major obstacle to introducing this method into routine clinical use. In this study, the accuracies of three different preparation methods for rapid direct identification of bacteria from positive blood culture bottles by MALDI-TOF MS analysis were compared.

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