Dried cerebrospinal fluid spots for diagnosing Japanese Encephalitis Virus (JEV) infection by Anti-JEV IgM antibody capture enzyme-linked immunosorbent assay: Harnessing the potential of a fully saturated pre-cut filter paper disc | Zendy

Tehmina Bharucha | Zendy; Onag Sengvilaipaseuth | Zendy; Anisone Chanthongthip | Zendy; E. Phuangpanom | Zendy; Ooyag Phonemixay | Zendy; Malavanh Vongsouvath | Zendy; S. Lee | Zendy; Paul N. Newton | Zendy; Audrey DubotPérès | Zendy

Open Access

Dried cerebrospinal fluid spots for diagnosing Japanese Encephalitis Virus (JEV) infection by Anti-JEV IgM antibody capture enzyme-linked immunosorbent assay: Harnessing the potential of a fully saturated pre-cut filter paper disc

Author(s) -

Tehmina Bharucha,

Onag Sengvilaipaseuth,

Anisone Chanthongthip,

E. Phuangpanom,

Ooyag Phonemixay,

Malavanh Vongsouvath,

S. Lee,

Paul N. Newton,

Audrey DubotPérès

Publication year - 2016

Publication title -

international journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.278

H-Index - 89

eISSN - 1878-3511

pISSN - 1201-9712

DOI - 10.1016/j.ijid.2016.02.364

Subject(s) - cerebrospinal fluid , medicine , japanese encephalitis , virology , spots , filter paper , antibody , dried blood spot , serial dilution , virus , encephalitis , immunology , pathology , chromatography , chemistry , alternative medicine

antigen (PGA) with nanoparticlessandwich ELISA, compared to the traditional sandwich ELISA Results: Sandwich ELISA achieved sensitivity of 93%, specificity of 92.5%, positive predictive value (PPV) of 95.7% and negativepredictive value (NPV) of 88%, while nano-sandwich ELISA achieved sensitivity, specificity, PPV and NPV of 95.8%, 95%, 97.2% and 92.6%, respectively. Conclusion: In conclusion, our research study provides that nano-sandwich ELISA isa well-established reference test for diagnosis of giardiasis.

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