The sensitivity to antibiotics of nosocomial strains of acinetobacter baumanii isolated in the tertiary hospitals in the Central Kazakhstan

Background: Acinetobacter baumanii are one of main bacterial pathogen caused nosocomial infection according International Guidelines of Infection Control (2015). Last 4 years the part of nosocomial infection caused by Acinetobacter baumanii are dramaticaly grows. Methods & Materials: In the multicenter study 200 strains of Acinetobacter baumanii were collected in period 20122015yy. Strains were collected in 3 tertiary hospitals in the Central Kazakhstan. All strains were identified by MALDI-TOF mass-spectrometry and typed by PCR detection of OXA-51 carbapenemase as A.baumanii specific label. The sensitivity testing were by micro dilution methods with CLSI criteria using. The OXA23 and OXA-40 carabapenemases genes detection made by PCR with commercial kits (Interlab Service, Russia). The statistical analysis (MIC90, average MIC, 95% Confidential Interval) was made by WhoNet 6.2 database. Results: All isolated strains are resistance to main part of antimicrobial drug (pic. 1). During fourth years period the resistance to carbapenems were increased: to imipenem 64,5%; 95%CI 45,5-80,2 (2012 year) to 81,2; 95%CI 66,8-90,5 (2015 year). The resistance growth by logarithmyc depence (y = 12,257ln(x) + 65,537; R2 = 0,9612). The testing of general linear hypothesis in regression situation for logarithmic model can predict level of resistance in 2016 at over 85% (pic.2). The dynamic of increasing to meropenem was the same and changed from 61.3% (2012) to 84,5% (2015y). In all cases of resistance to carbapenems the gene blaOXA-23 carbapenemase was detected. The quantitavive characteristics of