Molecular characterization of Leptospira sp by multilocus variable number tandem repeat analysis (MLVA) from clinical samples: a case report | Zendy

Hélène Pailhoriès | Zendy; Rodolphe Buzelé | Zendy; Mathieu Picardeau | Zendy; Sylvie Robert | Zendy; Emmanuelle Mercier | Zendy; Laurent Méreghetti | Zendy; Philippe Lanotte | Zendy

Open Access

Molecular characterization of Leptospira sp by multilocus variable number tandem repeat analysis (MLVA) from clinical samples: a case report

Author(s) -

Hélène Pailhoriès,

Rodolphe Buzelé,

Mathieu Picardeau,

Sylvie Robert,

Emmanuelle Mercier,

Laurent Méreghetti,

Philippe Lanotte

Publication year - 2015

Publication title -

international journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.278

H-Index - 89

eISSN - 1878-3511

pISSN - 1201-9712

DOI - 10.1016/j.ijid.2015.06.026

Subject(s) - multiple loci vntr analysis , variable number tandem repeat , leptospira , leptospira interrogans , fastidious organism , leptospirosis , genotype , biology , tandem repeat , virology , genetics , microbiology and biotechnology , bacteria , genome , gene

Leptospirosis is a zoonotic infection for which diagnosis is difficult. It has appeared as a global emerging infectious disease over recent years. Genotype determination often requires a Leptospira strain obtained by culture, which is a long and fastidious technique. A method based on multilocus variable number tandem repeat analysis (MLVA) to determine the genotype of Leptospira interrogans, performed directly on blood or urine samples, is proposed. This method was applied to a fatal case of leptospirosis for which the geographical origin of infection was unknown. This technique will allow a genotype to be obtained for L. interrogans, even when cultures remain negative.

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