ECC–RT-PCR: a new method to determine the viability and infectivity of Giardia cysts
Author(s) -
Absar Alum,
Basel Sbai,
Hamas Asaad,
Joseph R. Rubino,
Maryam Ijaz
Publication year - 2012
Publication title -
international journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 89
eISSN - 1878-3511
pISSN - 1201-9712
DOI - 10.1016/j.ijid.2012.01.004
Subject(s) - infectivity , giardia , biology , microbiology and biotechnology , virulence , viability assay , transcellular , cell culture , reverse transcription polymerase chain reaction , virology , messenger rna , gene , virus , genetics
Giardia sp is a major cause of diarrheal illness worldwide, and millions of people are infected each year. Rapid methods to determine the infectivity and virulence of isolates are critical for the development of intervention strategies to control the transmission of Giardia sp cysts, which occurs through contaminated surfaces, food, and water. However, determining the viability, infectivity, and virulence of Giardia sp cysts using molecular methods is a technical challenge because of the lack of a cell culture model.
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