Open AccessDistribution of Collagen Binding Proteins in Indian Isolates of Group A Streptococcus
Author(s) -
Chandana Priyanka,
Anuradha Chakraborti,
Saurabh Singh,
Rajesh Kumar
Publication year - 2008
Publication title -
international journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 89
eISSN - 1878-3511
pISSN - 1201-9712
DOI - 10.1016/j.ijid.2008.05.601
Subject(s) - bacterial adhesin , virulence , streptococcus , group a , microbiology and biotechnology , context (archaeology) , throat , pathogen , pathogenesis , rheumatic fever , streptococcus pyogenes , biology , gene , medicine , bacteria , immunology , genetics , staphylococcus aureus , anatomy , paleontology
Background: Streptococcus is an important human pathogen which causes a wide variety of diseases. Various virulence factors like adhesins, invasins and toxins are known to contribute to its pathogenesis. The adhesin like collagen binding proteins (CBPs) binds to host resulting in Streptococcal autoimmune sequel like rheumatic heart disease. So far, no study has been made on this context from India. In this study an attempt has been made to study the expression of different CBPs in Group A Streptococcus (GAS) strains from North India. Methods: Throat swabs were collected from the children (age group 5—15 yr) with the symptoms of rheumatic fever and confirmed for Group A Streptococcus. Strains were emm typed and studied for the presence of CBPs like cpa, cna, cne, scl1 and scl2 by PCR and real time-PCR analysis. Results: The prevalence of GAS is found to be 6.6 per thousand in our community. The strains showed the presence of emm types11, 12, 42, 57, 66, 68 and 81.1. Among the five CBP genes, three were present in all the throat isolates studied. The results were further confirmed with real time PCR. Conclusion: Prevalence of CBPs could be correlated with the virulence of GAS strains.
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