Open AccessA Novel Extended-Spectrum SHV-Type Beta-lactamase, SHV-104, From Klebsiella pneumoniae
Author(s) -
Nahed Ben Achour,
Paola Sandra Mercuri,
Cherifa Belhadj,
Mohamed Ben Moussa,
JeanMarie Frère,
Moreno Galleni,
Omrane Belhadj
Publication year - 2008
Publication title -
international journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 89
eISSN - 1878-3511
pISSN - 1201-9712
DOI - 10.1016/j.ijid.2008.05.286
Subject(s) - aztreonam , klebsiella pneumoniae , microbiology and biotechnology , cefotaxime , ceftazidime , imipenem , clavulanic acid , beta lactamase , biology , virology , amoxicillin , escherichia coli , antibiotic resistance , antibiotics , bacteria , pseudomonas aeruginosa , gene , genetics
codon83 (Serine83Arginine) was noted in 1 strain having a CIP MIC of 0.012 and a NA MIC of 32 g/ml. All 28 strains that had a CIP MIC of 0.016—1.5 g/ml and frank resistance to NA (MIC ≥16 g/ml; DD zone of inhibition ≤13 mm) carried a transversion mutation in codon 83 of gyrA (Serine83Isoleucine). We noted another transversion mutation in codon 85 of parC (Serine85Leucine) in 14 of these 28 strains that had a CIP MIC of ≥0.125—1.5 and a NA MIC ≥256 g/ml). We could not detect any mutations in gyrB (n = 24) and parE (n = 28) genes. Conclusion: Reduced CIP-susceptibility and NA-resistance of V. cholerae is associated with a gyrA mutation (Serine83Isoleucine). A further decrease in CIP-susceptibility is associated with parC mutation (Serine85Leucine).
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