Rapid diagnosis of rabies in humans and animals by a dot blot enzyme immunoassay | Zendy

Shampur Narayan Madhusudana | Zendy; Joel Paul | Zendy; Venugopal Karavattu Abhilash | Zendy; M. S. Suja | Zendy

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Rapid diagnosis of rabies in humans and animals by a dot blot enzyme immunoassay

Author(s) -

Shampur Narayan Madhusudana,

Joel Paul,

Venugopal Karavattu Abhilash,

M. S. Suja

Publication year - 2004

Publication title -

international journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.278

H-Index - 89

eISSN - 1878-3511

pISSN - 1201-9712

DOI - 10.1016/j.ijid.2004.02.006

Subject(s) - immunoassay , saliva , rabies , dot blot , antibody , rabies virus , western blot , biotinylation , virology , antigen , microbiology and biotechnology , chemistry , biology , immunology , biochemistry , dna , gene

The presently advocated tests for rapid diagnosis of rabies, such as the fluorescent antibody test (FAT) are expensive and require expertise to carry out and interpret the results. In this study, a simple direct dot blot enzyme immunoassay (DIA) has been developed and evaluated to detect the rabies antigen in brain specimens of animals and humans. The utility of this test in the ante-mortem diagnosis of human rabies has also been evaluated.

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