Open AccessHeterologous protein production in Zygosaccharomyces bailii : physiological effects and fermentative strategies
Author(s) -
Vigentini Ileana,
Brambilla Luca,
Branduardi Paola,
Merico Annamaria,
Porro Danilo,
Compagno Concetta
Publication year - 2005
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/j.femsyr.2004.11.006
Subject(s) - biology , heterologous , production (economics) , biochemistry , microbiology and biotechnology , gene , economics , macroeconomics
The optimisation and scale‐up of a specific protein production process have to take into account cultivation conditions as well as cell physiology of growth and the influence of foreign protein expression on host cell metabolism. The ability of Zygosaccharomyces bailii to tolerate high sugar concentrations as well as high temperatures and acidic environments renders this “non‐conventional” yeast suitable for the development of biotechnological processes like heterologous protein production. This work addresses the production of human interleukin‐1β by a recombinant Z. bailii strain. We found that the heterologous protein production causes some modifications of the Z. bailii carbon metabolism, leading to a reduced biomass yield. The other important factor is the dependence of the recombinant IL‐1β production/secretion on the growth rate. Among the cultivation strategies studied, the most appropriate in terms of production and productivity was the fed‐batch mode.