Open AccessYAP1 ‐mediated KNQ1 expression in Kluyveromyces lactis
Author(s) -
Imrichova Denisa,
Sarinova Marcela,
Cernicka Jana,
Gbelska Yvetta,
Subik Julius
Publication year - 2005
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/j.femsyr.2004.11.004
Subject(s) - biology , major facilitator superfamily , kluyveromyces lactis , transcription factor , gene expression , gene , saccharomyces cerevisiae , kluyveromyces , regulation of gene expression , microbiology and biotechnology , genetics , transporter
The b‐Zip transcription factor Yap1p plays an important role in oxidative stress response and multidrug resistance in Saccharomyces cerevisiae. We have previously demonstrated that the KNQ1 gene, encoding a multidrug transporter of the major facilitator superfamily in Kluyveromyces lactis and containing two potential Yap1p response elements in its promoter, is a putative transcriptional target of Kl Yap1p, the structural and functional homologue of Sc Yap1p. In this work, we provide evidence that KlYAP1 controls the expression of the KNQ1 gene. Using a P KNQ1 ‐ gusA fusion construct we showed that the expression of KNQ1 is induced upon cell treatment with the oxidizing agents H 2 O 2 and menadione and that this induction is mediated by Kl Yap1p. These results were confirmed by Northern‐blot analysis showing that the expression of KNQ1 is responsive to hydrogen peroxide and dependent on the presence of Kl Yap1p. The role of KlYAP1 in the control of KNQ1 expression was further demonstrated by EMSA experiments and drug resistance assays. These results clearly demonstrate the involvement of the Kl Yap1p transcription factor in the control of KNQ1 gene expression.