Open AccessHierarchical and metabolic regulation of glucose influx in starved Saccharomyces cerevisiae
Author(s) -
Rossell Sergio,
Weijden Coen C.,
Kruckeberg Arthur L.,
Bakker Barbara M.,
Westerhoff Hans V.
Publication year - 2005
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/j.femsyr.2004.11.003
Subject(s) - biology , yeast , starvation , function (biology) , metabolic regulation , biochemistry , flux (metallurgy) , carbon flux , saccharomyces cerevisiae , metabolic pathway , isozyme , microbiology and biotechnology , metabolism , enzyme , chemistry , ecology , endocrinology , organic chemistry , ecosystem
A novel method dissecting the regulation of a cellular function into direct metabolic regulation and hierarchical (e.g., gene‐expression) regulation is applied to yeast starved for nitrogen or carbon. Upon nitrogen starvation glucose influx is down‐regulated hierarchically. Upon carbon starvation it is down‐regulated both metabolically and hierarchically. The method is expounded in terms of its implications for diverse types of regulation. It is also fine‐tuned for cases where isoenzymes catalyze the flux through a single metabolic step.