Open AccessInvolvement of the yeast metacaspase Yca1 in ubp10 Δ‐programmed cell death
Author(s) -
Bettiga Maurizio,
Calzari Luciano,
Orlandi Ivan,
Alberghina Lilia,
Vai Marina
Publication year - 2004
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/j.femsyr.2004.07.005
Subject(s) - biology , saccharomyces cerevisiae , yeast , programmed cell death , caspase , ascorbic acid , apoptosis , microbiology and biotechnology , phenotype , deubiquitinating enzyme , biochemistry , gene , ubiquitin , food science
UBP10 encodes a deubiquitinating enzyme of Saccharomyces cerevisiae . Its inactivation results in a complex phenotype characterized by a subpopulation of cells that exhibits the typical cellular markers of apoptosis. Here, we show that additional deletion of YCA1 , coding for the yeast metacaspase, suppressed the ubp10 disruptant phenotype. Moreover, YCA1 overexpression, without any external stimulus, had a detrimental effect on growth and viability of ubp10 cells accompanied by an increase of apoptotic cells. This response was completely abrogated by ascorbic acid addition. We also observed that cells lacking UBP10 had an endogenous caspase activity, revealed by incubation in vivo with FITC‐labeled VAD‐fmk. All these results argue in favour of an involvement of the yeast metacaspase in the active cell death triggered by loss of UBP10 function.