Open AccessThe KlPGS1 gene encoding phosphatidylglycerolphosphate synthase in Kluyveromyces lactis is essential and assigned to chromosome I
Author(s) -
Tyciakova Silvia,
Obernauerova Margita,
Dokusova Lucia,
Kooistra Rolf A.,
Steensma H. Yde,
Sulo Pavol,
Subik Julius
Publication year - 2004
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/j.femsyr.2004.05.007
Subject(s) - biology , kluyveromyces lactis , gene , genetics , ura3 , microbiology and biotechnology , saccharomyces cerevisiae , nucleic acid sequence , mutant , coding region
The phosphatidylglycerolphosphate synthase (CDP‐diacylglycerol: sn ‐glycerol‐3‐phosphate 3‐phosphatidyltransferase, EC 2.7.8.5) is an essential enzyme in biosynthesis of cardiolipin. In this work we report the isolation, heterological cloning, molecular characterization and physical mapping of the Saccharomyces cerevisiae PEL1/PGS1 homologue from Kluyveromyces lactis. The pel1 mutant strain of S. cerevisiae was used to isolate this homologue by screening a K. lactis genomic library. The novel cloned gene was named KlPGS1. Its coding region was found to consist of 1623 bp. The corresponding protein exhibits 55% amino acid identity to its S. cerevisiae counterpart. The presence of the mitochondrial presequence indicates its mitochondrial localization. Sporulation and ascus dissection of diploids heterozygous for single‐copy disruption of KlPGS1 revealed that the KlPGS1 gene, is essential in K. lactis. Using a DIG‐dUTP‐labeled DNA probe‐originated from the KlPGS1 gene and Southern hybridization of contour‐clamped homogeneous electric field (CHEF)‐separated K. lactis chromosomal DNA, the KlPGS1 gene was assigned to chromosome I. The nucleotide sequence data reported in this paper were submitted to GenBank and assigned the Accession No. AY176328.