Open AccessPolyphasic identification of yeasts isolated from bark of cork oak during the manufacturing process of cork stoppers
Author(s) -
VillaCarvajal Mercedes,
Coque Juan José R.,
Luísa ÁlvarezRodríguez María,
Uruburu Federico,
Belloch Carmela
Publication year - 2004
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/j.femsyr.2004.01.007
Subject(s) - cork , biology , bark (sound) , rhodotorula , debaryomyces hansenii , botany , yeast , ecology , genetics
A two‐step protocol was used for the identification of 52 yeasts isolated from bark of cork oak at initial stages of the manufacturing process of cork stoppers. The first step in the identification was the separation of the isolates into groups by their physiological properties and RFLPs of the ITS‐5.8S rRNA gene. The second step was the sequencing of the D1/D2 domains of the 26S rRNA gene of selected isolates representing the different groups. The results revealed a predominance of basidiomycetous yeasts (11 species), while only two species represented the ascomycetous yeasts. Among the basidiomycetous yeasts, members representing the species Rhodosporidium kratochvilovae and Rhodotorula nothofagi , that have been previously isolated from plant material, were the most abundant. Yeasts pertaining to the species Debaryomyces hansenii var. fabryii , Rhodotorula mucilaginosa and Trichosporon mucoides were isolated in small numbers.