Open AccessOn the role of Trk1 and Trk2 in Schizosaccharomyces pombe under different ion stress conditions
Author(s) -
Calero Fernando,
Montiel Vera,
Caracuel Zaira,
CabelloHurtado Francisco,
Ramos José
Publication year - 2004
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1016/j.femsyr.2003.11.003
Subject(s) - schizosaccharomyces pombe , biology , strain (injury) , mutant , yeast , limiting , fission , schizosaccharomyces , hygromycin b , microbiology and biotechnology , gene , biochemistry , physics , mechanical engineering , quantum mechanics , neutron , engineering , anatomy
Trk1 and Trk2 are the major K + transport systems in Schizosaccharomyces pombe. Both transporters individually seem to be able to cope with K + requirements of the cells under normal conditions, since only the double mutant shows defective K + transport and defective growth at limiting K + concentrations. We have studied in detail the role of SpTrk1 and SpTrk2 under different ion stress conditions. Results show that the strain with only Trk1 ( trk1 + ) is less sensitive to Li + and to hygromycin B, it grows better at low K + and it survives longer in a medium without K + than the strain expressing only Trk2 ( trk2 + ). We conclude that Trk1 contributes more efficiently than Trk2 to the performance of the fission yeast under ion stress conditions. In the wild type both trk1 + and trk2 + genes are expressed and probably collaborate for the performance of the cells.