Open AccessGenetic aspects of targeted insertion mutagenesis in yeasts
Author(s) -
Klinner U,
Schäfer B
Publication year - 2004
Publication title -
fems microbiology reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.91
H-Index - 212
eISSN - 1574-6976
pISSN - 0168-6445
DOI - 10.1016/j.femsre.2003.10.002
Subject(s) - schizosaccharomyces pombe , mutagenesis , saccharomyces cerevisiae , biology , yeast , homologous recombination , genetics , schizosaccharomyces , genome , computational biology , gene , mutation
Targeted insertion mutagenesis is a main molecular tool of yeast science initially applied in Saccharomyces cerevisiae . The method was extended to fission yeast Schizosaccharomyces pombe and to “non‐conventional” yeast species, which show specific properties of special interest to both basic and applied research. Consequently, the behaviour of such non‐ Saccharomyces yeasts is reviewed against the background of the knowledge of targeted insertion mutagenesis in S. cerevisiae . Data of homologous integration efficiencies obtained with circular, ends‐in or ends‐out vectors in several yeasts are compared. We follow details of targeted insertion mutagenesis in order to recognize possible rate‐limiting steps. The route of the vector to the target and possible mechanisms of its integration into chromosomal genes are considered. Specific features of some yeast species are discussed. In addition, similar approaches based on homologous recombination that have been established for the mitochondrial genome of S. cerevisiae are described.