Open AccessFunctional coupling between vanillate‐ O ‐demethylase and formaldehyde detoxification pathway
Author(s) -
Hibi Makoto,
Sonoki Tomonori,
Mori Hideo
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.09.036
Subject(s) - pseudomonas putida , formaldehyde dehydrogenase , escherichia coli , strain (injury) , formate , demethylase , glutathione , formaldehyde , biochemistry , mutant , demethylation , chemistry , formate dehydrogenase , biology , gene , enzyme , gene expression , epigenetics , anatomy , dna methylation , catalysis
Pseudomonas putida vanillate‐ O ‐demethylase consisting of VanA and VanB was expressed in Escherichia coli strain K‐12. Recombinant E. coli strain K‐12 cells expressing VanAB efficiently converted vanillate into protocatechuate with glucose consumption. Mutant lacking either pgi or zwf showed higher or lower converting activity than the parental strain, respectively. Formaldehyde, which is the by‐product of the demethylation, was converted into formate in the cellular reaction. Formate accumulation was blocked by gene disruption of the E. coli frmA that coded glutathione‐dependent formaldehyde dehydrogenase.