Loop‐mediated isothermal amplification for the rapid detection of  Salmonella | Zendy

HaraKudo Yukiko | Zendy; Yoshino Manabu | Zendy; Kojima Tadashi | Zendy; Ikedo Masanari | Zendy

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Loop‐mediated isothermal amplification for the rapid detection of Salmonella

Author(s) -

HaraKudo Yukiko,

Yoshino Manabu,

Kojima Tadashi,

Ikedo Masanari

Publication year - 2005

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1016/j.femsle.2005.09.032

Subject(s) - loop mediated isothermal amplification , salmonella , salmonella enterica , serotype , microbiology and biotechnology , salmonella enteritidis , biology , enterobacteriaceae , bacteria , virology , chemistry , escherichia coli , gene , dna , genetics , biochemistry

Loop‐mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella . The sensitivity of the LAMP assay was found to be >2.2 cfu/test tube using nine serotypes. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was greater. Both fluorescence and turbidity were able to detect the products in the LAMP assay. S. enteritidis in a liquid egg sample artificially inoculated with the organism was detected by the LAMP assay at 2.8 cfu/test tube, although negative by PCR assay. These results indicate that the LAMP assay is a rapid, specific and sensitive detection method for Salmonella .

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