Open AccessHydrogenase genes are uncommon and highly conserved in Rhizobium leguminosarum bv. viciae
Author(s) -
Fernández Domingo,
Toffanin Annita,
Palacios José Manuel,
RuizArgüeso Tomás,
Imperial Juan
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.09.022
Subject(s) - rhizobium leguminosarum , biology , rhizobiaceae , gene , gene cluster , microbiology and biotechnology , genetics , strain (injury) , sequence analysis , bacteria , symbiosis , anatomy
A screening for h ydrogen up take ( hup ) genes in Rhizobium leguminosarum bv. viciae isolates from different locations within Spain identified no Hup + strains, confirming the scarcity of the Hup trait in R. leguminosarum. However, five new Hup + strains were isolated from Ni‐rich soils from Italy and Germany. The hup gene variability was studied in these strains and in six available strains isolated from North America. Sequence analysis of three regions within the hup cluster showed an unusually high conservation among strains, with only 0.5–0.6% polymorphic sites, suggesting that R. leguminosarum acquired hup genes de novo in a very recent event.