Open AccessConstruction of the first shuttle vectors for gene cloning and homologous recombination in Mycoplasma agalactiae
Author(s) -
ChopraDewasthaly Rohini,
Marenda Marc,
Rosengarten Renate,
Jechlinger Wolfgang,
Citti Christine
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.09.021
Subject(s) - biology , homologous recombination , cloning (programming) , shuttle vector , genetics , heterologous , gene , pathogen , complementation , vector (molecular biology) , recombinant dna , computational biology , phenotype , computer science , programming language
Mycoplasma agalactiae is a worldwide ruminant pathogen that causes significant economic losses by inflicting contagious agalactia in sheep and goats. The development of efficient control strategies requires a better understanding of the mycoplasma factors that promote successful infection. However, lack of genetic tools has been a major impediment in studying the pathogenic mechanisms of M. agalactiae . This study describes the identification and cloning of the M. agalactiae origin of replication ( oriC ) in order to construct the first shuttle vectors for targeted gene disruption, gene complementation and expression studies. Additionally, this report provides the first evidence of the occurrence of homologous recombination and the functionality of heterologous tetM determinant in this pathogen.