Open AccessIn situ analysis of native microbial communities in complex samples with high particulate loads
Author(s) -
Barra Caracciolo Anna,
Grenni Paola,
Cupo Cinzia,
Rossetti Simona
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.09.018
Subject(s) - in situ , fish <actinopterygii> , bacteria , fluorescence in situ hybridization , extraction (chemistry) , matrix (chemical analysis) , centrifugation , chromatography , aquifer , in situ hybridization , differential centrifugation , complex matrix , biology , chemistry , environmental chemistry , environmental science , microbiology and biotechnology , geology , groundwater , biochemistry , gene expression , geotechnical engineering , fishery , genetics , organic chemistry , gene , chromosome
In the present study a procedure combining a cell extraction method and Fluorescence In Situ Hybridization (FISH) for molecular monitoring and quantification of bacteria in soil and aquifer samples is presented. FISH was applied to bacterial cells extracted from the matrix by density gradient centrifugation. This separation method was applied to soil and aquifer samples and produced high cell recovery of 76.5%± 4.4 and 78.0%± 3.2, respectively. FISH, performed on the harvested cells, permitted a perfect visualization and quantification of bacteria. This approach is therefore promising for in situ detection of indigenous bacterial communities in complex samples.