Open AccessDetection of Vibrio parahaemolyticus in cockle ( Anadara granosa ) by PCR
Author(s) -
Bilung Lesley Maurice,
Radu Son,
Bahaman Abdul Rani,
Rahim Raha Abdul,
Napis Suhaimi,
Ling Michael Wong Clemente Vui,
Tanil Gwendelynne Bulan,
Nishibuchi Mitsuaki
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.08.053
Subject(s) - vibrio parahaemolyticus , cockle , biology , hemolysin , microbiology and biotechnology , polymerase chain reaction , most probable number , virulence , gene , bacteria , fishery , genetics
This study aimed to determine the occurrence of Vibrio parahaemolyticus in cockles ( Anadara granosa ) at a harvesting area and to detect the presence of virulent strains carrying the thermostable direct hemolysin ( tdh ) and TDH‐related hemolysin genes ( trh ) using PCR. Of 100 samples, 62 were positive for the presence of V. parahaemolyticus with an MPN (most probable number) value greater than 3.0 (>1100 MPN per g). The PCR analysis revealed 2 samples to be positive for the tdh gene and 11 to be positive for the trh gene. Hence, these results demonstrate the presence of pathogenic V. parahaemolyticus in cockles harvested in the study area and reveal the potential risk of illness associated with their consumption.