Open AccessProduction of exopolysaccharide from mycelial culture of Grifola frondosa and its inhibitory effect on matrix metalloproteinase‐1 expression in UV‐irradiated human dermal fibroblasts
Author(s) -
Bae Jun Tae,
Sim Gwan Sub,
Lee Dong Hwan,
Lee Bum Chun,
Pyo Hyeong Bae,
Choe Tae Boo,
Yun Jong Won
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.08.021
Subject(s) - grifola frondosa , mycelium , chemistry , matrix metalloproteinase , food science , microbiology and biotechnology , photoaging , matrix (chemical analysis) , collagenase , interstitial collagenase , biochemistry , polysaccharide , biology , botany , chromatography , enzyme , genetics
Exopolysaccharide (EPS) was prepared by submerged mycelial culture of a newly isolated mushroom Grifola frondosa HB0071 in a 5‐l stirred‐tank fermenter. This fungus produced a high concentration of biomass (24.8 g l −1 at day 4), thereby achieving high EPS concentration (7.2 g l −1 at day 4). EPS was proven to be a proteoglycan consisting of 85.6% carbohydrates (mostly glucose) and 7.3% proteins with a molecular weight of 1.0 × 10 6 Da. The photoprotective potential of EPS was tested in human dermal fibroblasts (HDF) exposed to ultraviolet‐A (UVA) light. It was revealed that EPS had an inhibitory effect on human interstitial collagenase (matrix metalloproteinase, MMP‐1) expression in UVA‐irradiated HDF without any significant cytotoxicity. The treatment of UVA‐irradiated HDF with EPS resulted in a dose‐dependent decrease in the expression level of MMP‐1 mRNA (by maximum 61.1% at an EPS concentration 250 μg ml −1 ). These results suggest that EPS obtained from mycelial culture of G. frondosa HB0071 may contribute to inhibitory action in photoaging skin by reducing the MMP 1‐related matrix degradation system.